Microwave Peptide Synthesizer Liberty Prime 2.0

The Liberty PRIME™ 2.0 is the absolute best peptide synthesizer available in the world. The system features a unique one-pot deprotection and coupling process allowing for a remarkable 2 minute cycle time with only 8.5mL waste per cycle (at 0.1mmol). A key improvement in the 2.0 version of the Liberty PRIME is the ability to make cleaner and longer peptides with a new level of robustness than previously possible.

Features

• Unprecedented 2.5 min cycle time (at 0.1mmol)
• Unrivaled 8.5mL waste per cycle (at 0.1mmol)
• Precise calibration-free delivery of all reagents
• True Internal fiber-optic temperature control
• 0.005 - 5 mmol scale range w/ digital N2 control
• 30 amino acid positions
• Integrated RV Camera
• High-throughput upgrade options (HT4, HT12, HT24)
• Ability to use in cGMP peptide production

Unmatched Quality

A new level of peptide purity.

The Liberty PRIME 2.0 provides the ability to make even higher purity peptides than previously possible with the original Liberty Blue system. This is based on advances in the system fluidics, reaction vessel environment, and chemistry methodology.

• Optimized microwave chemistry methodology with ProTide resins
• Cleanest reaction environment with updated headspace flushing technology
• More direct optimized flow paths
• Digitally variable N2 control for optimized bubbling, purging, and draining

Barstar Length: 89 amino acids Synthesis Time: 11 h 26 min Waste Generated: 1660 mL Scale: 0.05 mmol

HIV Protease Length: 99 amino acids Synthesis Time: 12 hrs. 48 min. Waste Generated: 1829 mL Scale: 0.05 mmol

MDM2 Length: 127 amino acids Synthesis Time: 16 hrs. 26 min. Waste Generated: 2316 mL Scale: 0.05 mmol

Unrivaled Speed

Solvent

Advanced Reagent Delivery System

CarboMAX

• The fastest and most complete coupling
• Stabilizes acid sensitive linkages which are problematic for standard carbodiimide chemistry (phospho, glyco, hyper-acid linkages)
• Unmatched low-level of epimerization for all 20 amino acids due to faster activation (faster o-acylisourea formation)

High Throughput (HT) Loaders

High-throughput peptide synthesis made easy.

• Automated synthesis of 4, 12, or 24 peptides (individual 20mer’s can be completed every hour)
• The Liberty PRIME 2.0 paired with either the HT4, HT12, or HT24 outperforms conventional parallel systems 

Software

Flexible, Powerful Software

The newly updated software on the Liberty PRIME 2.0 is both flexible and easy to use, making sure nothing stands between you and your synthesis. Customize methods with unique monomers, orthogonal deprotection, branching, cyclizing, and more. Easily check on reactions on the run screen, where the run can be edited, paused, and resumed at any time.

Easy and Flexible Sequence Programming

The method editor on the Liberty PRIME 2.0 allows full customization of reaction parameters.

Program Post-Synthesis Modification

Versatile options enabling orthogonal deprotection for modifications like branching and cyclization.

Full Suite of Calculator Tools

Convenient calculators provide the reagents and amounts for a run.

Pause, Edit, Monitor at any Step

Easily pause, edit, and monitor reactions from the run screen.

Audit Trail

Equipped with audit trail, the Liberty Prime 2.0 software time/date stamps all changes, runs, maintenance operations, etc.

21 CFR Part 11 Compliant

The Liberty Prime 2.0 software is 21 CFR part 11 compliant, ready for cGMP laboratory environments.

Applications

Synthesize standard and complex peptides.

The Liberty 2.0 series, like preceding models, utilizes CEM’s High-Efficiency Solid Phase Peptide Synthesis (HE-SPPS) and CarboMAX coupling methodologies,

• Branched Peptides
• Cyclized Peptides
•  Disulfide Bonding
•  Glycopeptides (N/O-linked)
• High Throughput Synthesis
• N-Methyl Peptides
•  Peptoids
• Phosphopeptides