Automated In Situ Hybridization and Immunohistochemistry System InsituPro

The InsituPro is a dedicated system for automated in situ hybridization (ISH) to prepare samples for detection of DNA or RNA(mRNA, miRNA) and also for immunohistochemistry (IHC) to detect proteins and other antigens. The system automates all of the tedious and repetitive washing and incubation steps for ISH and IHC processes.

Features

• Works with whole mounts, vibratome sections, thin sections on slides, and cells on coverslips
• Used on wide range of organisms (mouse, zebrafish, chicken, Xenopus, Drosophila, Arabidopsis, and many more)
• Fully customizable programs
• 16 buffer positions (2 cooled for preserving delicate antibodies)
• Allows partial recovery of expensive antibodies and probes 

Flexibility

Use Whole Mounts and Slides

The InsituPro easily accommodates both slides and whole mount samples. Easily switch between samples in only minutes. Up to 60 whole mounts/vibratome sections or slides can be processed in parallel with individual probes or antibodies.

Baskets	Slides
60 x 5mm inner diameter (Medaka, Drosophila, Xenopus, small vibratome sections)	60 x (75mm x 25mm x 1.0mm)
60 x 9mm inner diameter (chicken, mouse, medium vibratome sections)	60 x (75mm x 25mm x 1.0mm)
32 x 13mm inner diameter (large vibratome, large specimens)	60 x (75mm x 25mm x 1.0mm)

Whole mount preparations and vibratome sections are processed in separate incubation baskets in keyhole shaped cavities in the specimen tray. The specimen tray allows for heating and cooling between 6°C – 75°C and provides very gentle liquid exchange for even the most delicate samples of the specimens.

Slides with thin sections are mounted on a counter slide forming an independent incubation chamber. Six of these chambers are combined to a slide block and ten of these blocks are mounted vertically into the heated incubation tub. The incubation tub allows for control of temperature and high humidity for optimal performance. Liquid exchange is driven by gravity flow and capillary forces ensure the sections are submerged in buffer.

Established System

Widely Used

The InsituPro has a strong record for In situ Hybridization and Immunohistochemistry.

• Used at > 300 research groups worldwide
• Cited in > 550 publications
• Applicable to zebrafish, mouse, Ababidopsis, Drosophila, Xenopus, chicken, and more

Open System

Low Running Costs

The InsituPro does not require the use of special kits and allows for standard lab buffers to be used. Additionally, the system allows for partial recovery of expensive probes and antibodies that can then be reused for subsequent samples. These features make operating the InsituPro very cost efficient.

Flexible Software

Easily Adaptable Protocols

The InsituPro comes with ready to use protocols for a wide range of species. However, each protocol is easily adaptable and allows full control of all parameters including temperature settings and parallel processing of samples with different incubation times. Individual treatment of each samples is also possible including the use of unique antibodies and probes.